bodog体育beijingshijian8yue13ri，bodogtiyushengmingxueyuansunboketizuzaizhimingxueshuqikan《embo reports》shangzaixianfabiaotiwei“dynamics of staphylococcus aureus cas9 in dna target association and dissociation”（jinhuangseniangnongputaoqiujuncas9yumubiaodnajiehejijielidedonglixue）deyanjiulunwen。gailunwenshoucijieshilejinhuangseniangnongputaoqiujunzhongdecas9（staphylococcus aureus cas9，sacas9）danbaiyubabiaodnajiehe、jiexuan、qiegeyijijielizheiyidongtaiguochengdefenzijizhiyijiliangzhezhijiandewendinghuzuoweidian。gaigongzuoduisacas9zuoweidnagongjudekaifaheyingyongjuyouzhongyaodezhidaoyiyi。
bodog体育crispr-casxitongshicunzaiyuxijunjigujunzhongdeyizhongshiyingxingmianyifangyuxitong，nenggoudiyuwaiyuanhesuanderuqin，weichijiyindewanzhengxinghewendingxing。gaixitongzhongdezhongyaocasdanbaishibiejiangezixianglinjixu（protospacer adjacent motif, pam），zaizhidaorna (guide rna, grna）deyindaoxiapipeimubiaodnahouduiqijinxingqiege。crispr-casxitongdejiandan、linghuohegaoxiaoshiqizuoweiyizhongxindejiyinzugongjuzaigezhongshengwutizhongyouzheguangfanyingyong。erzuoweiyixiangxinjishu，crispr-casxitongdeyingyongtongyangyecunzaiyixiejidaijiejuedewenti，rucasdanbaicunzaituobaxiaoying，feibaxiangqiegekenengfashengzaiduishengwugongnengyouzhongyaoyingxiangdejiyinzuweizhi，congerpohuaixibaodejibengongneng。zheijigaixitongdeyingyongdailailejidafengxian。ershenrulejiecasdanbaidegongzuojizhi，duitigaoqizuoweidnagongjudexiaolvhezhunquelvjianghuiyoujudabangzhu。
sacas9shimuqianziranjiezhongfaxiandeiixingcrispr-casxitongzhongtijijiaoweixiaoqiaodecas9danbai，you1053geanjisuanzucheng。yinci，gaidanbaibianyutineidisong，kezaiduozhongshengwuzhongjinxingjiyinzubianji。gaiyanjiushiyongyixiliededanfenzifangfaxitongdiyanjiulesacas9yubaxiangdnahuzuodedonglixuejizhi（tu1a）。yanjiufaxian，sacas9jieheheqiegebaxiangdnafenbiexuyao6bphe18bpdepamjinduandnayugrnahubu。zheixiemeihuoxingshiyoudnadanbaijianlianggewendingdehuzuoweidianjiedaode，qizhongzhiyiweiyujupamyue6bpchu，lingyigezaiyuanshijiangequyunei（tu1b）。youqudeshi，sacas9zaiqiegednahouhuizidongshifangpamyuanduandna，tongshibaochiyupamdejiehe（tu1b）。gaibufendnadeshifangtongshixiaochulesacas9yuyuanshijiangezidnadeqiangxianghuzuoyong，youyiyuqisuihoucongpamduandezuizhongjieli。
zheixianggongzuoweijinyibuyouhuasacas9huoxing、jianshaotuobatigonglezhongyaodefenzijichu，bingqieweishejigrnayigaijinqiteyixinghekaifagenggaoxiaolvdesacas9yanshengwutigonglexinsilu。
gailunwenzhong，sunbojiaoshouketizu2018jiboshiyanjiushengzhangsiqihe2020jieboshishengzhangqian（yibiye）weigongtongdiyizuozhe，sunbojiaoshouweibenwenweiyitongxunzuozhe，shangkedaweidiyiwanchengdanwei。gaiyanjiugongzuodedaolekejibu、shanghaishikeweiyijishangkedakeyanqidongjijindezhichi。
wenzhanglianjie:
tu1: (a) danfenziyingguangguangniejishuguancesacas9qiegednahoushifangbufendna；(b) sacas9jiehe、jiexuan、qiegeyijishifangbaxiangdnadedongtaiguochengshiyitu。
