生命学院孙博课题组解析SaCas9蛋白与靶向DNA互作的分子机制

ON2019-08-14CATEGORY科研进展

bodog体育beijingshijian8yue13ri,bodogtiyushengmingxueyuansunboketizuzaizhimingxueshuqikan《embo reports》shangzaixianfabiaotiwei“dynamics of staphylococcus aureus cas9 in dna target association and dissociation”(jinhuangseniangnongputaoqiujuncas9yumubiaodnajiehejijielidedonglixue)deyanjiulunwen。gailunwenshoucijieshilejinhuangseniangnongputaoqiujunzhongdecas9(staphylococcus aureus cas9,sacas9)danbaiyubabiaodnajiehe、jiexuan、qiegeyijijielizheiyidongtaiguochengdefenzijizhiyijiliangzhezhijiandewendinghuzuoweidian。gaigongzuoduisacas9zuoweidnagongjudekaifaheyingyongjuyouzhongyaodezhidaoyiyi。

bodog体育crispr-casxitongshicunzaiyuxijunjigujunzhongdeyizhongshiyingxingmianyifangyuxitong,nenggoudiyuwaiyuanhesuanderuqin,weichijiyindewanzhengxinghewendingxing。gaixitongzhongdezhongyaocasdanbaishibiejiangezixianglinjixu(protospacer adjacent motif, pam),zaizhidaorna (guide rna, grna)deyindaoxiapipeimubiaodnahouduiqijinxingqiege。crispr-casxitongdejiandan、linghuohegaoxiaoshiqizuoweiyizhongxindejiyinzugongjuzaigezhongshengwutizhongyouzheguangfanyingyong。erzuoweiyixiangxinjishu,crispr-casxitongdeyingyongtongyangyecunzaiyixiejidaijiejuedewenti,rucasdanbaicunzaituobaxiaoying,feibaxiangqiegekenengfashengzaiduishengwugongnengyouzhongyaoyingxiangdejiyinzuweizhi,congerpohuaixibaodejibengongneng。zheijigaixitongdeyingyongdailailejidafengxian。ershenrulejiecasdanbaidegongzuojizhi,duitigaoqizuoweidnagongjudexiaolvhezhunquelvjianghuiyoujudabangzhu。

sacas9shimuqianziranjiezhongfaxiandeiixingcrispr-casxitongzhongtijijiaoweixiaoqiaodecas9danbai,you1053geanjisuanzucheng。yinci,gaidanbaibianyutineidisong,kezaiduozhongshengwuzhongjinxingjiyinzubianji。gaiyanjiushiyongyixiliededanfenzifangfaxitongdiyanjiulesacas9yubaxiangdnahuzuodedonglixuejizhi(tu1a)。yanjiufaxian,sacas9jieheheqiegebaxiangdnafenbiexuyao6bphe18bpdepamjinduandnayugrnahubu。zheixiemeihuoxingshiyoudnadanbaijianlianggewendingdehuzuoweidianjiedaode,qizhongzhiyiweiyujupamyue6bpchu,lingyigezaiyuanshijiangequyunei(tu1b)。youqudeshi,sacas9zaiqiegednahouhuizidongshifangpamyuanduandna,tongshibaochiyupamdejiehe(tu1b)。gaibufendnadeshifangtongshixiaochulesacas9yuyuanshijiangezidnadeqiangxianghuzuoyong,youyiyuqisuihoucongpamduandezuizhongjieli。

zheixianggongzuoweijinyibuyouhuasacas9huoxing、jianshaotuobatigonglezhongyaodefenzijichu,bingqieweishejigrnayigaijinqiteyixinghekaifagenggaoxiaolvdesacas9yanshengwutigonglexinsilu。

gailunwenzhong,sunbojiaoshouketizu2018jiboshiyanjiushengzhangsiqihe2020jieboshishengzhangqian(yibiye)weigongtongdiyizuozhe,sunbojiaoshouweibenwenweiyitongxunzuozhe,shangkedaweidiyiwanchengdanwei。gaiyanjiugongzuodedaolekejibu、shanghaishikeweiyijishangkedakeyanqidongjijindezhichi。

wenzhanglianjie:

tu1: (a) danfenziyingguangguangniejishuguancesacas9qiegednahoushifangbufendna;(b) sacas9jiehe、jiexuan、qiegeyijishifangbaxiangdnadedongtaiguochengshiyitu。